| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Thorpe, Olivia R (9)
|
SCRAYS |
41.69 |
40.68 |
-1.01 |
| 2 |
Ono, Haruka (10)
|
SCRAYS |
42.09 |
41.28 |
-0.81 |
| 2 |
Ullman, Elise O (10)
|
NCMSA |
41.22 |
41.28 |
0.06 |
| 4 |
Meehan, Amelia M (10)
|
SCRAYS |
44.24 |
41.76 |
-2.48 |
| 5 |
Druckemiller, Lily A (10)
|
SCRAYS |
44.43 |
45.73 |
1.30 |
| 6 |
Basara, Grace M (10)
|
SCRAYS |
50.00 |
47.02 |
-2.98 |
| 7 |
Babani, Elsa N (9)
|
NCATOM |
48.58 |
48.47 |
-0.11 |
| 8 |
Wilkerson, Amelia H (10)
|
SCRAYS |
47.52 |
48.48 |
0.96 |
| 9 |
Ivan, Dominique (9)
|
NCMSA |
55.86 |
49.63 |
-6.23 |
| 10 |
Murphy, Anna J (9)
|
NCATOM |
51.99 |
49.75 |
-2.24 |
| 11 |
Faulkenberry, Madison B (10)
|
SCRAYS |
49.47 |
50.08 |
0.61 |
| 12 |
Bullins, Logan A (9)
|
NCATOM |
|
50.12 |
- |
| 13 |
Schutzman, Eleanora R (10)
|
SCRAYS |
56.60 |
51.40 |
-5.20 |
| 14 |
Higbee, Addison S (10)
|
NCATOM |
|
51.59 |
- |
| 15 |
Yang, Avery J (8)
|
NCATOM |
51.56 |
52.18 |
0.62 |
| 16 |
Korn, Emma M (9)
|
NCATOM |
54.57 |
52.37 |
-2.20 |
| 17 |
Eckert, Hannah G (10)
|
SCRAYS |
|
53.09 |
- |
| 18 |
Kodakandla, Amukta (10)
|
SCRAYS |
|
54.75 |
- |
| 19 |
Roelandts, Samantha D (8)
|
SCRAYS |
|
56.73 |
- |
| 20 |
Taylor, Riley J (9)
|
NCATOM |
57.57 |
56.96 |
-0.61 |
| 21 |
Dawson, Isabella M (8)
|
NCATOM |
1:04.41 |
57.18 |
-7.23 |
| 22 |
Hennessy, Madeline G (9)
|
NCATOM |
|
57.39 |
- |
| 23 |
Shaw, Blakely B (8)
|
NCATOM |
1:04.31 |
1:02.13 |
-2.18 |
| 24 |
Gaza, Lauren E (7)
|
SCCS |
|
1:05.69 |
- |
| 25 |
Hightower, Maci C (8)
|
SCRAYS |
|
1:07.62 |
- |
| 26 |
Walter, Aspen S (8)
|
SCCA |
|
1:24.96 |
- |
|
Knox, Virginia E (10)
|
NCATOM |
|
DQ |
- |